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KMID : 1234420090370030243
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Korean Journal of Microbiololgy and Biotechnology
2009 Volume.37 No. 3 p.243 ~ p.247
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Lee Seung-Ha
Lee Eun-Hee
Cho Kyung-Suk
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Abstract
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The usage of ethanol (EtOH)-blended gasoline (gasohol), has been increasing in recent years. EtOH has influence on the distribution and biodegradation of aromatic compounds such as BTEX (benzene (B), toluene (T), ethylbenzene (B), and xylene (X)) that are gasoline compositions. In this study, the effect of EtOH on the aerobic biodegradation of B, T and E was investigated using a BTEX and EtOH-degrading bacterium, Rhodococcus sp. EH831. The degradation rates of B in
the conditions of 1:1, 1:4, and 1:0.25 mixtures with EtOH (B:EtOH, mol:mol) were ranged from 3.82±0.20 to 5.00±0.37 μmol · g-dry cell wight (DCW)-1 · h-1. The degradation rate of T was the fastest in the 1:0.25 mixture (6.63±0.06 μmol · g-DCW-1 · h-1), and it was the lowest in the 1:4 mixture (4.41±0.04 μmol ·DCW-1 · h-1). The degradation rates of E were increased with increasing the addition amount of EtOH: The degradation rate of E was the highest in the 1:4 mixture (1.60±0.03 μmol · g-DCW-1 · h-1), and the rates were 1.42±0.06, 1.30±0.01, and 1.01±0.30 μmol · g-DCW-1 · h-1 in the 1:1, 1:0.25, 1.0 mixtures, respectively. In conclusion, the biodegradation of B, T, E by Rhodococcus sp. EH831 was not significantly inhibited by the co-existence of EtOH.
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KEYWORD
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Gasohol, benzene, toluene, ethylbenzene, ethanol, aerobic biodegradation
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